Fig. 6
Blocking autophagic flux reversed the anti-apoptosis and antioxidation effects of EF-sEVs in vitro. A Representative images showing the levels of ROS in each group. Scale bar=1000 μm. B Quantitative analysis of levels of ROS in PC12 cells in each group. C The apoptosis rates of PC12 cells were determined using flow cytometry. D Percentages of apoptotic cells in each group. E The viability of H2O2-exposed PC12 cells were detected using CCK-8 assays. F Western blot analysis of autophagy-related and apoptosis-related proteins. G Quantitative analysis of autophagy-related and apoptosis-related protein expression levels. Data are expressed as mean ± SD (n = 3)