Fig. 7
LncRNA-MALAT1 was a key component of EF-sEVs-induced neuroprotection. A Volcano plots of the results of RNA sequencing and differential lncRNA expression profile analysis. Blue indicates decreased expression and red indicates increased expression of the dysregulated lncRNAs in EF-sEVs (p < 0.05). The gray indicates no significant change. B Heatmap of differentially expressed lncRNAs. The top 10 upregulated lncRNA, lncRNA-MALAT1 (red box) increases most significantly. C, D Quantitative real-time PCR of lncRNA-MALAT1 in normal and EF condition in hucMSCs and sEVs, respectively. E Representative images show the hucMSCs transfected with shRNA or shRNA negative control (green). Scale bar: 100 μm. F qRT-PCR for lncRNA-MALAT1 in hucMSCs and sEVs after transfection. G, H Flow cytometry analysis of apoptosis percentage in PC12 cells treated with shRNA-EF-sEVs. I CCK-8 assay cell viability of H2O2-induced PC12 cells co-cultured with PBS, shMALAT1-EF-sEVs, and shMALAT1-NC-EF-sEVs. J, K Protein expression levels of autophagy-related proteins and apoptosis-related proteins, presented as the average expression normalized to β-actin levels. Data are expressed as mean ± SD (n = 3)