Fig. 8

LncRNA-MALAT1 exerted neuroprotective effects by sponging miR-22-3p. A The binding site of lncRNA-MALAT1 and miR-22-3p. B Luciferase activity assay of HEK293T cells transfected with luciferase constructs containing mimics and inhibitor of lncRNA-MALAT1. C–E H₂O₂-induced PC12 cells were transfected with miR-22-3p mimics, miR-22-3p inhibitor, and their negative controls. Cell viability was assessed using CCK-8 assay (C). Cellular apoptosis was analyzed using flow cytometry (D, E). F, G Protein expression levels of Bcl-2, cleaved caspase 3, Beclin1, and p62 from H₂O₂-induced PC12 cells transfected with miR-22-3p mimics, miR-22-3p inhibitor, and their negative controls. Data are expressed as mean ± SD (n = 3)