Fig. 9
miR-22-3p enhanced AMPK phosphorylation in PC12 cells by targeting SIRT1. A The binding site of miR-22-3p and SIRT. B, C Luciferase activity assay of HEK293T cells transfected with luciferase constructs containing mimics and inhibitor of SIRT1. D Levels of SIRT1 and phosphorylated AMPK in H2O2-induced PC12 cells treated with shMALAT1-EF-sEVs and shMALAT1-NC-EF-sEVs. E SIRT1 and phosphorylated AMPK levels in PC12 cells treated with miR-22-3p mimics, miR-22-3p inhibitor, and their negative controls. F, G SIRT1 levels in H2O2-induced PC12 cells treated with different miR-22-3p versions in different concentrations