Fig. 3
Ca2+ overload, PTT and ROS overproduction result in a synergistic enhancement of the cytotoxicity of SCCI nanoparticles. A Cytotoxicity of 4T1 cells treated with increasing concentrations of various formulations. Data are shown as mean ± SD (n = 3). B Hemolysis rate of different samples after incubation with RBC. C Fluorescence intensity analysis of DCFH-DA in different treated 4T1 cells with 808 nm laser irradiation. (0.75 W/cm2, 3 min). Data are shown as mean ± SD (n = 3). ****P < 0.0001. D Calcein-AM/PI live/dead double staining of 4T1 cells incubated with Cur, CCI, SCCI. Untreated cells served as the control group, and SCCI + L group was irradiated with an 808 nm laser after administration (0.75 W/cm2, 3 min). Scale bar, 100 μm. E Fluorescence images of DCFH-DA in 4T1 cells treated with different formulations after 808 nm laser irradiation (0.75 W/cm2, 3 min). Scale bar, 100 μm