Fig. 4

SCCI releases CUR and Ca²⁺ by consuming H⁺ in lysosomes. A SCCI in lysosomes for different time treatment to 4T1 cells. Scale bar: 20 μm. B Fluorescence microscope images of intracellular Ca²⁺ production after 6 h treatment with CCI and SCCI. SCCI + L group was irradiated with an 808 nm laser after administration (0.75 W/cm², 3 min). Scale bar, 100 μm. C The mean fluorescence intensity of intracellular Ca²⁺ production quantified by flow cytometry. Data are shown as mean ± SD (n = 3). ****P < 0.0001