Fig. 5
In vitro intracellular uptake and MMP detection. A CLSM of the uptake of Cur@CaCO3-RhB and SA/Cur@CaCO3-RhB by 4T1 cells. Scale bar, 75 μm. B Cellular uptake of C6, Cur@CaCO3-C6 and SA/Cur@CaCO3-C6 quantified by flow cytometry. C Analysis of the cellular uptake rate of C6, Cur@CaCO3-C6 and SA/Cur@CaCO3-C6 in 4T1 cells. Data are shown as mean ± SD (n = 3). ****P < 0.0001. D Analysis of intracellular ATP content after treatment with different formulations. Data are shown as mean ± SD (n = 3). ***P < 0.001, ****P < 0.0001. E MMP detection of 4T1 cells treated with different preparations. CCI + L group and SCCI + L group were irradiated by 808 nm laser after administration. Scale bar, 50 μm