Fig. 2

Healthy plasma-derived exosomes reduce sciatic nerve injury in DPN. A Representative immunofluorescent staining images of sciatic nerve axons (green) and myelin (red) in different groups, scale bar = 200/50 µm. Squares, images enlarged in right panel. B Statistical histogram of the proportion of demyelinating axons in different groups, n = 3. C The ultrastructure of sciatic nerves was observed by TEM in different groups of rats, scale bar = 5/2 µm. The yellow arrows point to the site of abnormal demyelination. D Quantitative of G-ratio in different groups, n = 10. E Representative images showing PGP9.5 positive intraepidermal nerve fibers in the posterior plantar skin of different groups of rats, scale bar = 100 μm. F The IENFD analysis is shown in F, n = 5. Data are presented as the mean ± SEM. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)