Fig. 4

Plasma-exos augments the motor and sensory innervation of the targets. A A schematic of NMJ and muscle spindle. B Representative images of NMJ, labeled with 555-a-BTX (red) and NF-L + Syn (green) in different groups, bar = 100/50 µm. Squares, images enlarged as shown below. C Statistical analysis of the proportion of innervated NMJ (partial denervation and total denervation are excluded), n = 6. D Representative images of annulospiral endings of muscle spindles in different groups, bar = 200 μm. E Number of muscle spindles in different IRD distribution intervals. F Representative images of different groups of neurons labeled with NeuN (red) and retrograde tracer FITC-CTB (green), bar = 400 μm. G Statistical analysis of the proportion of FITC-CTB positive cells, n = 3. H Statistical analysis of wet weight of gastrocnemius muscle in each group, n = 3. I Changes of miR-20b-3p expression in sciatic nerve of rats in each group (G), n = 4. Data are presented as the mean ± SEM. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)