Fig. 5

MiR-20b-3p agomir improve nerve damage induced by high glucose. A A schematic showing the process of miR-20b-3p agomir administration. B–D Changes in Von Frey test, plantar test and rotarod test after the treatment of DPN rats with miR-20b-3p stable N.C. or miR-20b-3p agomir, n = 5. E, F Changes of MCV and SCV after miR-20b-3p agomir treatment, n = 3, 6. G The ultrastructure of sciatic nerves was observed by TEM in different groups of rats, scale bar = 5 μm/2µm. The new myelin sheath is marked with *. H Histogram represents the quantitative data of the G-ratio under various conditions, n = 10. I Representative images showing PGP9.5 positive intraepidermal nerve fibers in the posterior plantar skin of different groups of rats, scale bar = 100 μm. J Statistical analysis of IENFD is shown in J, n = 5. K Representative images of Dil perfusion in different groups of transverse and longitudinal section. Transverse section, scale bar = 200 μm and longitudinal section, scale bar = 1000 μm. L Statistical analysis of Dil (red) fluorescence area ratio in transverse section, n = 3. M Representative images of plantar blood flow imaging in different groups of rats. N Statistical analysis of plantar blood perfusion per unit area is shown in N, n = 5. B–D Data are presented as the mean ± SD, others are presented as the mean ± SEM. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)