Fig. 6

MiR-20b-3p agomir augments the motor and sensory innervation of the targets. A Representative images of NMJs labeled with 555-a-BTX (red) and NF-L + Syn (green) in different groups, scale bar = 100/50 µm. Squares, images enlarged in right panel. B Statistical analysis of the proportion of innervated NMJs (partial denervation and total denervation are excluded), n = 6. C Representative images of annulospiral endings of muscle spindles in different groups, scale bar = 200 μm. D Number of muscle spindles in different IRD distribution intervals. E Representative images of different groups of neurons labeled with NeuN (red) and retrograde tracer by CTB (green), scale bar = 500 μm. F Statistical analysis of the proportion of CTB positive cells, n = 4. G Statistical analysis of wet weight of gastrocnemius muscle in each group, n = 5. H Red fluorescently labeled agomir was found in the NF-L-labeled sciatic nerve. Data are presented as the mean ± SEM. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)