Fig. 7

Plasma-exos improves RSC96 autophagy under high glucose stimulation. A Bubble map for KEGG analysis of miR-20b-3p. B Chordal graph of GO enrichment analysis of target genes of miR-20b-3p in autophagy related pathways. (GO:0006914 autophagy, GO:0061919 process utilizing autophagic mechanism, GO:0010506 regulation of autophagy, GO:0061912 selective autophagy, GO:0010508 positive regulation of autophagy, GO:0010507 negative regulation of autophagy, GO:0000422 autophagy of mitochondrion). C Venn diagram of the 115 matched targets between the diabetes, autopaghy and peripheral nerve targets. D Grey strips by western blot of the expression of Stat3, pStat3, Atg10, LC3 II/I at different groups in RSC96 in vitro after stimulation with hplasma-exos. E–H Quantification of the related expression of Stat3 (E), pStat3 (F), Atg10 (G) and LC3 II/I (H) in RSC96 after stimulation with PBS or hplasma-exos, n = 3. I TEM images of autophagy organelles in RSC96 cells with different stimuli, bar = 2 μm. Yellow arrows, autophagy organelles. J Statistical analysis of the number of autophagy organelles in indicated groups, n = 8–10. K Grey strips by western blot of the expression of Stat3, pStat3, Atg10, LC3 II/I at different groups in RSC96 in vitro. L–O Statistical results of the expression of Stat3 (L), pStat3 (M), Atg10 (N) and LC3 II/I (O) at different groups in RSC96 after different stimuli, n = 3–4. HG high glucose environment, LG low glucose environment. Data are presented as the mean ± SEM. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)