Fig. 8

MiR-20b-3p down-regulates the expression of Stat3 and promotes autophagy in Schwann cells. A TEM images of autophagy organelles in RSC96 cells with different stimuli, bar = 2 μm. Yellow arrows, autophagy organelles. B Statistical analysis of the number of autophagy organelles in indicated groups, n = 8–12. C Grey strips by western blot of the expression of Stat3, pStat3, Atg10, LC3 II/I at different groups in RSC96 in vitro. D–G Statistical results of the expression of Stat3 (D), pStat3 (E), Atg10 (F) and LC3 II/I (G) at different groups in RSC96 after different stimuli, n = 3. H TEM images of autophagy organelles in RSC96 cells with different stimuli, bar = 2 μm. Yellow arrows, autophagy organelles. I Statistical analysis of the number of autophagy organelles in indicated groups, n = 8–12. J Complementary sequences between miR-20b-3p and the 3′-untranslated region (UTR) of wild type or mutant type of Stat3 were obtained. K Target relationship between miR-20b-3p and Stat3 was assessed by dual luciferase reporter gene assay. L–P Quantitative analysis and statistical results of the expression of Stat3, pStat3, Atg10, LC3 II/I at different groups in vivo by Western blot, n = 3. HG high glucose environment, LG low glucose environment. Data are presented as the mean ± SEM. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)