Fig. 3
From: Zn-Shik-PEG nanoparticles alleviate inflammation and multi-organ damage in sepsis
Modulation of the AMPK/SIRT1/NLRP3 inflammatory signaling pathway. (A and B) RT-qPCR analysis of the expression of TNF-α and IL-6. (C) Representative western blotting bands of AMPK, p-AMPK, SIRT1, NLRP3. (D and E) Representative immunofluorescence images of SIRT1 and NLRP3 in RAW264.7 cells after different treatments. (Nucleus: blue; SIRT1: red; NLRP3: green). (F) Representative western blotting bands of ASC, cleaved caspase-1, caspase-1, IL-1β, IL-18. (G) Representative TUNEL staining from different treatment of RAW264.7 cells. (Nucleus: blue; TUNEL: green). Scale bar: 20 μm. *p < 0.05, ** p < 0.01, and ***p < 0.001