Fig. 5

GAP43 mediated the protection of Hyp-sEVs on MNU-induced apoptosis of 661 W cells. (A, B) Western blot for the knockdown efficiency of GAP43 in Hyp-MSCs and Hyp-sEVs after transfection. (C) Western blot for the expressions of PCNA, Bcl-2 and Bax in 661 W cells. (D) CCK8 assay for the proliferation of 661 W cells (n = 5). (E) Immunofluorescent staining images of 661 W cells showing Ki-67 expression. Scale bars, 25 μm. (F) TUNEL staining of 661 W cells. Scale bars, 25 μm. (G) Immunofluorescent staining images of 661 W cells showing Cleaved Caspase-3 expression. Scale bars, 25 μm. (H) Cell apoptosis assay of 661 W cells (n = 3). All data are presented as means ± SEM. ns, not significant, *P < 0.05, and ***P < 0.001