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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: A novel “prime and pull” strategy mediated by the combination of two dendritic cell-targeting designs induced protective lung tissue-resident memory T cells against H1N1 influenza virus challenge

Fig. 2

Dendritic cells (DCs) targeting Salmonella stimulated DC differentiation in vitro and in vivo. The BMDCs were prepared and incubated with Salmonella strains χ11802 (pYL180) and χ11802 (pYL230), named S180 and S230, respectively, with a multiple of infection (MOI) of 100 for 2 h. BSG and LPS (0.5 μg/mL) were also included as mock and positive controls. The maturation markers MHC-II, CD40 and CD86 were determined by flow cytometry (A). An illustration of the animal experiment (Study 1) is shown (B). One day after the second immunization, PPs (C), MLNs (D) and spleens (E) were collected and analyzed by flow cytometry to evaluate the expression levels of MHC-II, CD40 and CD86 (n = 4, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, not significant)

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Journal of Nanobiotechnology

ISSN: 1477-3155

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