Fig. 9

Generation of CD4⁺ and CD8⁺ lung-resident memory T (T_RM) cells in lung tissues in Study 2. At 30 days post-3rd immunization in Study 2, single-cell suspensions from the lung samples were collected and subjected to FACS to determine the percentages of CD4⁺CD44⁺CD69⁺ (A), CD4⁺ CD44⁺CD69⁺CD103⁺ (B), CD4⁺ CD44⁺CD69⁺CD103⁻ (C), CD8⁺CD44⁺CD69⁺ (D), CD4⁺ CD44⁺CD69⁺CD103⁺ (E) and CD8⁺ CD44⁺CD69⁺CD103⁻ (F) T_RM cells. The intracellular production of IL-17 was also determined (G). (n = 4, *P < 0.05, **P < 0.01, ***P < 0.001)