Table 1 Bacterial strains, plasmids and virus used
Materials | Description | Source |
|---|---|---|
Bacteria strains | ||
χ11802 | Regulated delayed lysis Salmonella | Roy Curtiss III University of Florida |
χ6212 | E.coli host strain with asd mutation | Roy Curtiss III University of Florida |
Top10 | E.coli host strain for regular cloning | Kangti, China |
BL21 | E.coli host strain for protein expression and purification | Kangti, China |
Plasmids | ||
pYA3681 | Prokaryotic expression vector in Salmonella, arabinose dependent | Roy Curtiss III University of Florida |
pYL180 | pYA3681 based prokaryotic vector, express 3M2e-ferritin fusion protein | Wang et al. [38] |
pYL230 | pYL180 based prokaryotic vector, express 3M2e-ferritin fusion protein, as well as pgsA’-scFv-CD11c sequence | This study |
pYL139 | PgsA’ anchored scFv-CD11c on surface of E.coli or Salmonella | This study |
pET28a | Prokaryotic expression vector, kanamycin resistant | Lab stock |
pYL272 | pET28a based vector expressing 3M2e-ferritin, His tag | This study |
pYL273 | pET28a based vector expressing DCpep3-3M2e-ferritin, His tag | This study |
pYL285 | pEASY-blunt based standard plasmid harboring M1 gene of H1N1 | Lab stock |