Fig. 3

Cellular internalization against HUVECs. A Representative fluorescence microscopy image of HUVECs incubated with Ox-CS/C6 NPs (green) for different times (1, 3, 6 h). Nuclei were stained with DAPI (blue), while cell membrane were stained with DiI (red), scale bar = 100 μm. B Representative peak graph and C quantification of mean fluorescence intensity (MFI) in HUVECs after incubation with Ox-CS/C6 NPs for varied times performed by flow cytometry. n ≥ 3 per group. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001