Fig. 4
In vitro anti-oxidant and anti-inflammatory capacity of Ox-CS/CeRT NPs. A Intracellular ROS of H2O2-stimulated HUVECs after different interventions for 12 h was observed via fluorescent microscopy, scale bar = 300 μm. B–D The mRNA levels of pro-inflammatory cytokines in HUVECs after LPS treatment and different interventions for 4 h. Cytokine gene expression was normalized to GAPDH. E Semi-quantitative results of intracellular ROS. F Cell viability of H2O2-stimulated HUVECs after incubation with RT, Ox-CS/Ce NPs, Ox-CS/CeRT NPs for 24 h. n ≥ 3 per group. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001