Fig. 2

Characterization and tracking of ADSCs. (a) A schematic of the isolation of ADSCs. ADSCs isolated from perirenal adipose tissues of rats were cultured in vitro. (b) The recruitment effect of SDF1-α on ADSCs via SDF1-α/CXCR4 axis was tested by Transwell assay (ADM3100, a CXCR4 antagonist to block SDF1-α/CXCR4 signal transduction; nucleus in blue), and the quantified data was presented in (c) (n = 8, * indicated P < 0.05, compare to the Cont. group, # indicated P < 0.05). Scale bar, 100 μm. (d) ADSCs were labeled by incubating with EdU for various times (24 and 48 h) and the labeling efficacy of ADSCs was assessed by EdU staining (EdU positive in red, nucleus in blue), and the quantified data was presented in (e) (n = 7, * indicated P < 0.05). Scale bar, 200 μm. (f) The tracking and recruitment of ADSCs were conducted in vivo. ADSCs incubated with EdU for 48 h were harvested and injected into the penises of 8-week-old rats with various regents (PBS, BP, SDF1-α, and BP@SDF1-α, the dose of BPNS and SDF1-α were equal to BP@SDF1-α). The detection of EdU positive cells in penis tissues at different time points (1 d, 2 d, and 3 d) was visualized by laser confocal microscope (EdU positive in red, nucleus in blue), and the quantified data was presented in (g) (n = 6, * indicated P < 0.05, compared to the PBS group, # indicated P < 0.05, ns means no significance). Scale bar, 200 μm