Fig. 4

The recruitment effect of SDF1-α on endogenous S/PCs in vivo. (a) A schematic of detecting the recruitment of endogenous S/PCs. The recruitment effects of SDF1-α on endogenous S/PCs in corpus cavernosum and MPG were assessed. Intraperitoneal injections of EdU (50 mg/Kg) into new-born rats were conducted and various regents (PBS, BP, SDF1-α and BP@SDF1-α, the dose of BPNS and SDF1-α were equal to BP@SDF1-α) were injected into the penis and the base and periphery of MPG respectively. (b) The recruitment effect of SDF1-α on endogenous S/PCs in the penis was assessed. The penis tissues were harvested at different time points (1, 2, 3, 7, and 14 days post various reagents injection) and the recruitment efficacy was visualized by EdU staining of penis tissues (EdU positive in red, nucleus in blue, white arrows points to the EdU positive cells). Scale bar, 200 μm. The quantified data was presented in (c) (n = 6, * indicated P < 0.05, compared to the PBS group, # indicated P < 0.05). (d) The MPG tissues were harvested at different time points (1, 2, and 3 days post various reagents injection) and the recruitment efficacy was visualized by EdU staining of MPG tissues (EdU positive in red, nucleus in blue, white arrows points to the EdU positive cells). Scale bar, 200 μm. The quantified data was presented in (e) (n = 6, * indicated P < 0.05, compared to the PBS group, # indicated P < 0.05)