Fig. 4

BMS-1 potentiated CNH-PG-mPT’s toxicity to tumor cells. A Effect of CNH-PG on 4T1 cell viability. B Effects of LI and CNH-PG-mPT on 4T1 cell viability. C Effects of CNH-PG and CNH-PG-mPT on the protein levels of PD-L1, caspase 3, BCL2, and BAX. The gray analysis results of caspase 3, BCL2, and BAX are presented in Additional file 1: Fig. S3. D Effects of CNH-PG and CNH-PG-mPT on 4T1 cell surface PD-L1 abundance. E Effects of CNH-PG-mPT, BMS-1, and their combination on 4T1 cell viability. Effects of CNH-PG, BMS-1, CNH-PG plus BMS-1, CNH-PG-mPT, and CNH-PG-mPT plus BMS-1 on F, G 4T1 cell proliferation and death; H protein levels of PD-L1, caspase 3, BCL2, and BAX; and I 4T1 cell surface PD-L1 abundance. Cell viability was assayed using a CCK-8 kit. Protein levels were assayed by western blotting. Cell surface PD-L1 abundance was assayed via immunofluorescent staining and flow cytometry. Cell proliferation was assayed via CFSE staining and flow cytometry. Cell death was assayed via annexin V staining and flow cytometry. Values are means ± standard deviation (SD). (n = 3, # & *p < 0.05, ## & **p < 0.01). Representative flow cytometry dot plots/histograms are provided in Additional file 1: Fig. S4