Fig. 5

BMS-1 potentiated CNH-PG-mPT to skew TAMs into an M1-like phenotype. A Effects of CNH-PG and CNH-PG-mPT on M2 cell viability. Effects of CNH-PG, BMS-1, CNH-PG plus BMS-1, CNH-PG-mPT, and CNH-PG-mPT plus BMS-1 on B protein content of PD-L1 and PD-1 in M2; C cell surface PD-L1 abundance in M2. The gray analysis result of PD-1 is presented in Additional file 1: Fig. S3; D–F cell surface abundance of CD80, CD86, and CD206 in M2; G protein content of ARG1, iNOS, and GBP5; and H, I phagocytic activity of M2. Cell viability was assayed using a CCK-8 kit. Protein levels were assayed by western blotting. Cell surface protein abundance was assayed via immunofluorescent staining and flow cytometry. The phagocytic function was assayed using fluorescent latex beads and flow cytometry. Cell death was assayed via annexin V staining and flow cytometry. Values are means ± standard deviation (SD). (n = 3, # & *p < 0.05, ## & **p < 0.01). Representative flow cytometry dot plots/histograms are provided in Additional file 1: Fig. S5