Fig. 6

BMS-1 synergized with CNH-PG-mPT to skew TAMs in co-culture with 4T1 cells into an anti-tumor M1-like phenotype. Effects of CNH-PG, BMS-1, CNH-PG plus BMS-1, CNH-PG-mPT, and CNH-PG-mPT plus BMS-1 on A–C and F cell surface abundance of PD-L1, CD86, CD80, and CD206 in M2; and D, E phagocytic activity of M2. G Cell surface abundance of PD-L1 in co-cultured 4T1 cells. H, I Proliferation and death of co-cultured 4T1 cells. Cell surface protein abundance was assayed via immunofluorescent staining and flow cytometry. The phagocytic function was assayed using fluorescent latex beads and flow cytometry. Cell proliferation was assayed via CFSE staining and flow cytometry. Cell death was assayed via annexin V staining and flow cytometry. Values are means ± standard deviation (SD). (n = 3, # & *p < 0.05, ## & **p < 0.01). Representative flow cytometry dot plots/histograms are provided in Additional file 1: Fig. S6. Normalized data of TAM phenotyping and 4T1 toxicity from Figs. 4, 5, 6 showing the synergy of BMS-1 with CNH-PG-mPT in single culture and co-culture are presented in Additional file 1: Table S1 for comparison