Fig. 2

Target behavior of NO^·@PLT in vitro and in vivo. (A) SEM images of NO^·@PLT during the activation process. (B) The nano NO^· micelles release curve from NO^·@PLT during the activation. (C) Experimental schematic of the Transwell system (0.4-µm pore size). The NO^·@PLT was added to the upper layer, and U87 cells were cultured in the lower layer. (D) Fluorescence images of U87 cells in the lower chamber. NO^·@PLT was modified with Cy 5.5. (E) Fluorescence analysis of fluorescently labeled NO^·@PLT in the lower layer. (F) In vivo images of mice bearing U87 tumors treated with NO^·@PLT at 0 h (before) and 1.5 h (after). (G) Tumor site in vivo fluorescence intensity quantification after intravenous injection at 0 h (before) and 1.5 h (after). Data are presented as the mean ± SD (n = 5)