Fig. 3

Characterization of trehalose-releasing nanogels (TNG). (A) Section of ¹H NMR spectra of TreA (top) and TNG (bottom) proving covalent incorporation of trehalose into TNG (D₂O, 600 MHz). (B) Cryo-TEM micrograph of TNG in water. Scale bar: 100 nm. (C) Colloidal stability of TNG in DMEM + 10% FBS monitored by DLS measurement. (D) Trehalose release profile from TNG in PBS over one month (pH 7.4, 37 °C, TNG concentration: 100 µg/mL). Data are presented as mean ± SD, n = 3