Fig. 6
AS/LIG/AS_LIG@PPGC NPs inhalation reverses lung fibrosis caused by BLM through NOX4-NLRP3 signalling pathway. (A to C). Representative molecular docking of AS-IV and LIG with NOX4 and NLRP3 proteins, respectively. (A). Molecular docking of AS-IV with NOX4. (B). Molecular docking of LIG with NOX4. (C). Molecular docking of AS-IV with NLRP3. (D, E). Western blotting analysis of expression and quantification of proteins related to NOX4-NLRP3 signalling pathway (NOX4, NLRP3, pro-caspase-1, caspase-1, ASC, IL-1β, and IL-18) in the lung sections on day 22 treated as indicated. (F, G). Western blotting analysis of expression and quantification of proteins related to NOX4-NLRP3 signalling pathway (NOX4, NLRP3, pro-caspase-1, caspase-1, ASC, IL-1β, and IL-18) in the cells treated as indicated. (H, I). IHC staining analysis of lung sections and quantification of proteins related to NOX4-NLRP3 signalling pathway on day 8. Scale bar = 200 μm. (H). IHC staining analysis of lung sections of NOX4, NLRP3. (I). Quantification of proteins of NOX4, NLRP3. (J, K). IHC staining analysis of lung sections and quantification of positive area of proteins related to NOX4-NLRP3 signalling pathway on day 22. Scale bar = 200 μm. (J). IHC staining analysis of lung sections of NOX4, NLRP3. (K). Quantification of positive area of NOX4, NLRP3. * P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, n.s., not significant, P > 0.05