Fig. 7

AS/LIG/AS_LIG@PPGC NPs inhalation reverses lung fibrosis caused by BLM through NOX4-p38 MAPK signalling pathway. (A, B). IHC staining analysis of lung sections and quantification of proteins related to NOX4-p38 MAPK signalling pathway on day 8, 22. Scale bar = 200 μm. (A). IHC staining analysis of lung sections of p-p38 MAPK. (B). Quantification of positive area of p-p38 MAPK. (C, D). Western blotting analysis of expression and quantification of proteins related to NOX4-p38 MAPK signalling pathway (NOX4, p38, and p-p38) in the lung sections on day 22 treated as indicated. (E, F). Western blotting analysis of expression and quantification of proteins related to NOX4-p38 MAPK signalling pathway (NOX4, p38, and p-p38) in the cells treated as indicated. (G-K). Expression levels of typical pro-inflammatory and profibrotic cytokines (TNF-α, IL-6, IL-1β, and TGF-β1) in BALF induced by BLM after different treatments at day 8 (G), and day 22 (K). The results were expressed as the mean ± SD (n = 3). * P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, n.s., not significant, P > 0.05