Fig. 8
RNA-seq analysis. (A, B). Volcano plot of DE genes in transcription phase. The cutoff was set as log2(fold change) > 1 and adjusted p value < 0.05. The fold changes and p values were calculated by DESeq2. (C). Heatmap of the expression of the DE genes associated with model, AS_LIG@ PPGC groups. (D). The gene sets that were significantly enriched are arranged into categories of biological processes, cellular components, and molecular functions, and are presented in a bar plot. (E). Bubble plot of the KEGG pathway analysis enriched by differentially expressed genes between the model and AS_LIG@PPGC NPs groups. The colors of the nodes reflect the p-values of the designated pathways, and the sizes of the nodes indicate the number of differentially expressed genes enriched in the pathways. (F). GSEA analysis plot based on differentially expressed genes between the model and AS_LIG@PPGC NPs groups