Fig. 6

A. tumefaciens-mediated co-expression of NbCRVP and NbCalB enhances the antiviral effect. a Effects of 35S::CRVP and 35S::CalB co-expression on TMV infection at 2 and 4 dpi, as measured by RT-qPCR, with 35S::00 and 35S::CRVP as the control treatments. The data were analyzed by Duncan’s multiple range tests in the ANOVA program of SPSS. Different letters indicate significant differences among the four treatment groups (P < 0.05). b Effects of 35S::CRVP and 35S::CalB co-expression on TMV infection at 4 dpi, as measured using WB. c Effects of 35S::CRVP and 35S::CalB co-expression on biological symptoms between the three different treatments