Fig. 3

Axon outgrowth of NSCs and DRGs in vitro on various materials. (A) Confocal microscopy images showing the expression of neurofilament (NF, red) and β3-tubulin (Tuj-1, Green) in NSCs after 7 days of culture on different samples. Nuclei were stained with Hoechst (blue). Scale bar = 100 μm. (B) Quantification of axon density and axon length in NSCs grown on various materials (n = 3). (C) Gene expression profiles of NSCs cultured on different samples for 7 days (n = 3). (D) Immunofluorescence (IF) images of NF-positive axons in DRGs grown on different samples for 7 days. Scale bar = 200 μm. (E) Quantitative analysis of axon density and length in DRGs (n = 3). (F) Expression of GAP43 and NF proteins in NSCs cultured on different samples for 7 days. (G) Quantification of Western blot data (n = 3). Statistical analysis was performed using ANOVA followed by Tukey’s test (*p < 0.05, **p < 0.01, and ***p < 0.001)