Fig. 5

Endogenous neurogenesis is enhanced by TPME implantation. (A) Representative photograph of NeuN (green) staining 8 weeks after SCI in different experimental groups. Scale bar = 500 μm. (B) Quantitative analysis of the area occupied by NeuN-positive cells (n = 3). (C) Immunofluorescence images from the various groups at 8 weeks. Neuronal markers (Tuj-1, green) and glial fibrillary acidic protein (GFAP, red) were used to stain the spinal cords. Scale bar = 500 μm. (D) Quantitative study of Tuj-1 and GFAP immunofluorescence intensity in spinal cord tissue (n = 3). (E) Tuj-1 and GFAP protein expression. (F) Quantitative analysis of Tuj-1 and GFAP in Western blotting studies (n = 3). Statistical analysis was performed using ANOVA followed by Tukey’s test (*p < 0.05, **p < 0.01, and ***p < 0.001)