Fig. 7

Mechanism of TPME hydrogels in promoting axon regeneration after SCI. (A) The expression of PTEN/PI3K/AKT/mTOR pathway proteins was detected using Western blotting. (B) Western blotting assays were used to quantitatively analyze PTEN, p-PI3K, p-AKT, p-mTOR, and p-P70S6K (n = 3). (C) A schematic illustration demonstrates that the mechanism by which TPME hydrogels promote axon regeneration is through the activation of the PTEN/PI3K/AKT/mTOR pathway. Statistical analysis was performed using ANOVA followed by Tukey’s test (*p < 0.05, **p < 0.01, and ***p < 0.001)