Fig. 3
From: Encapsulation and assessment of therapeutic cargo in engineered exosomes: a systematic review
Laboratory detection and evaluation of engineered exosomes. After harvesting drug-loaded exosomes, the total protein is first quantified to determine exosome yield. Next, UV absorbance of the exosome suspension is measured to quantify drug loading efficacy. The engineered exosomes are then fluorescently labeled, enabling evaluation of their biodistribution and drug release capabilities in vitro and in vivo. Finally, storage conditions are optimized by studying the engineered exosomes after freezing for various periods