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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: A colorimetric, photothermal, and fluorescent triple-mode CRISPR/cas biosensor for drug-resistance bacteria detection

Fig. 3

Optimization of experimental conditions. (a) The effects of BSA encapsulation on absorbance. (b) The concentration of HRP on absorbance. (c) The amounts of magnetic beads on absorbance. (d) The trans-cleavage time on absorbance. (e) the laser power density on ΔT of the platform. (f) the irradiation time on ΔT of the platform. ΔT (℃) = ΔT (Target+)- ΔT (Target-), ΔT (Target+) is the temperature change after laser irradiation upon targets, ΔT (Target-) is the temperature change after laser irradiation upon no target. (g) The trans-cleavage time on fluorescent intensity. (h) The TdT reaction time on fluorescent intensity. (i) The usage of magnetic beads on fluorescent intensity

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