Fig. 3

Changes of cell subsets proportions and biological features in PT and DCT cells upon PS-MPs plus HFD treatment. A UMAP of PT cells colored by four treatment groups (left) and PT cell subsets (right). B The proportion of PT cell subsets in the ND and HFD groups (left) and four treatment groups (right). C Heatmap of the top 10 DEGs among PT cell subsets according to log fold changes. D Violin plot of the top 3 DEGs among PT cell subsets according to log fold changes. E KEGG enrichment analysis of upregulation pathways of PT1_1 cells in PS-MPs group vs. ND group. F KEGG enrichment analysis of upregulation pathways of GlomerularEpi cells in PS-MPs group vs. ND group. G Pseudotime trajectory analysis colored by PT cell subsets at varying pseudotime stages (up) and by individual PT cell subsets (down). H UMAP of DCT cells colored by four treatment groups (left) and PT cell subsets (right). I The proportion of DCT cell subsets in the ND and HFD groups (left) and four treatment groups (right). J Heatmap of the top 10 DEGs among DCT cell subsets according to log fold changes. K Violin plot of the top 3 DEGs among DCT cell subsets according to log fold changes. L KEGG enrichment analysis of upregulation pathways of DCT_1, DCT_3, and DCT_4 in PS-MPs group vs. ND group. M Pseudotime trajectory analysis colored by DCT cell subsets at varying pseudotime stages (up) and individual DCT cell subsets (down)