Fig. 4

Changes in the biological features and differentiation trajectories of ECs upon PS-MPs plus HFD treatment. A UMAP of endothelial cells colored by four treatment groups (left) and PT cell subsets (right). B The proportion of endothelial cell subsets in the four treatment groups. C Heatmap of the top 10 DEGs among endothelial cell subsets according to log fold changes. D Violin plot of the top 3 DEGs among endothelial cell subsets according to log fold changes. E KEGG enrichment analysis of upregulation pathways of CapECs in PS-MPs group vs. ND group. F KEGG enrichment analysis of upregulation pathways of CapECs in PS-MPs plus HFD group vs. HFD group. G Pseudotime trajectory analysis colored by endothelial cell subsets at varying pseudotime stages (left) and by individual endothelial cell subsets (right). H The top 8 differential expression genes with pseudotime progression