Fig. 2

Cell assays. (a) Confocal fluorescence microscope images of 4T1 cancer cells treated by Hochest (blue fluorescence), Mito-tracker (green fluorescence) and Pd@Au-Cy5.5 (red fluorescence) to demonstrate their mitochondria-targeting ability (Scale bar: 10 μm). (b) The cell-level biocompatibility of Pd@Au-PEG NSs. (c) Cells viabilities of 4T1 cancer cells after different treatments for 24 h. (d) Western blotting images of HIF-1α expression of 4T1 cancer cells after different treatments for 24 h under the hypoxic culture condition. (e) Fluorescence microscopy images of 4T1 cancer cells dual-stained with Calcein-AM (green fluorescence) and PI (red fluorescence) after different treatments for 24 h (Scale bar: 50 μm)