Fig. 2

BTFPTU inhibits the proliferation and migration of osteosarcoma cells in vitro. (A) Viability of HOS cells with PTU, TFPTU or BTFPTU treatment for 24 h using Cell Counting Kit-8 assay. (B) Effect of PTU, TFPTU or BTFPTU treatment for 48 h on the colony-forming ability of HOS cells based on colony formation assays. Scale bar, 200 μm. (C) Quantification of the colony number. (D) HOS cells were treated with different concentrations of BTFPTU for 24 h, and the cell proliferation was characterized by the EdU assay. Scale bar, 200 μm. (E) Flow cytometry to determine apoptosis of HOS and 143B cells with or without BTFPTU (2 µM) treatment for 12 h by Annexin V-FITC/PI apoptosis detection. (F) Quantification of apoptosis rate of HOS and 143B cells. (G) Cell migration abilities of HOS and 143B cells treated with different concentrations of BTFPTU for 24 h were measured by Transwell migration assays. Scale bar, 200 μm. (H) Quantification of the migration abilities of HOS and 143B cells. (I) The wound healing assay was used to evaluate the migration abilities of HOS cells treated with different concentrations of BTFPTU for 24 h. Scale bar, 50 μm. (J) Quantification of the wound closure rate. (K) The protein expression of N-Cadherin, Vimentin and c-Myc were measured by Western blot analysis in HOS cells treated with varying concentrations of BTFPTU for 24 h. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated