Fig. 6

OTP-BTFPTU liposomes suppresses osteosarcoma tumorigenesis and metastasis in vivo. (A) Living fluorescence imaging of subcutaneous xenograft tumor model mice, which were injected with 0.1 mL of saline or OTP-BTFPTU liposomes (5 × 10 − 5 M) through tail vein. The imaging was performed 24 h after injection (n = 4). (B) Quantification of the relative fluorescence of the nude mice which were injected with saline or OTP-BTFPTU liposomes. (C) Living luminescence imaging of subcutaneous xenograft tumor model mice with saline or OTP-BTFPTU liposomes treatment (n = 4). (D) Quantification of the relative luminescence of subcutaneous xenograft tumor model mice with saline or OTP-BTFPTU liposomes treatment. (E) Photographs of HOS derived xenograft model with saline or OTP-BTFPTU liposomes treatment (n = 4). (F) Tumor volume of different groups was measured every 7 days after mice were injected with HOS cells. (G) The average tumor weight in each group when the mice were sacrificed. (H) The expression of c-Myc, E-cadherin and N-cadherin were determined by immunohistochemistry. Scale bars, 400 μm. (I) HE staining of heart, kidney, liver, lung and spleen which were obtained from different groups. Scale bars, 300 μm. The data represent the mean ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 for a comparison with the control group or as indicated