Fig. 4
From: Nano-carrier DMSN for effective multi-antigen vaccination against SARS-CoV-2
The DMSN-P-R vaccine elicits a strong neutralizing antibody response. (A) Schematic of the immunization design. Mice were initially immunized on Day 0, followed by boosters on Day 14 and 28. Sera were collected on Day10 post immunization. (B) Sera from immunized mice were analyzed for RBD-specific IgG antibodies using ELISA. (C, D) Neutralizing antibodies were assessed using live SARS-CoV-2 virus (Wuhan and Delta strain). (E) The purified RBDOmicron of SARS-CoV-2 was analyzed by SDS-PAGE. (F) Sera were analyzed for RBDOmicron-specific IgG antibodies using ELISA. (G) Neutralizing antibodies were assessed using live SARS-CoV-2 Omicron BA.1 virus. (B–D, F, G) The data are presented as the mean ± s.e.m. A nonparametric Mann-Whitney test was used for the statistical analysis. **p < 0.01