Fig. 5

The DMSN-P-R vaccine elicits SARS-CoV-2 specific Th1 and Th2 immune responses. (A, B) Sera from immunized mice were used to determine the IgG subclasses by ELISA. (C, D) Mouse splenocytes were isolated on Day 10 post immunization. The ELISPOT assay was performed to measure IFN-γ (C) and IL-4 (D) secretion from splenocytes following stimulation with the RBD peptides pool. (E) The ELISPOT assay was performed to measure IFN-γ secretion from splenocytes following stimulation with conserved T-cell peptides pool. (A–E) The data are presented as the mean ± s.e.m. A nonparametric Mann-Whitney test was used for the statistical analysis. *p < 0.05, **p < 0.01