Fig. 6

Safety evaluation of DMSN vaccine. (A) The viability of THP-1 and 293T cells incubated with the DMSN at various concentrations was assessed using CCK-8. (B–E) The DMSN was inoculated into BALB/c mice via intramuscular injection. (B) The body weight (relative to the initial body weight) of the mice was measured. Fifteen days AfterDMSN inoculation, the serum ALT (C) and AST (D) were measured. (E) H&E staining images of mouse organs were analyzed. Scale bars,50 μm. (C, D) The data are presented as the mean ± s.e.m. A nonparametric Mann-Whitney test was used for the statistical analysis. n.s., not significant (p ≥ 0.05)