Fig. 3
a Schematic diagram of PTT/PDT process. Cell viability of b 4T1 cells, c HeLa cells and d B16F10 cells incubated with different concentrations of PYT NPs with and without 808 nm (1.0 W cm−2) laser irradiation for 5 min. b–d n = 4, “ns” means “no significance”. ****P < 0.0001, t-test. Calcein AM (green)/propidium iodide (red) double-staining fluorescence images of e 4T1, f HeLa cells and g B16F10 cells treated with PBS, NIR, PYT NPs, and PYT NPs + NIR. Scale bar: 100 μm