Table 1 Chemicals, peptides and Recombinant Proteins
From: Enhancing immune responses of ESC-based TAA cancer vaccines with a novel OMV delivery system
| Â | Reagent or resource antibody | Source | Identifier |
|---|---|---|---|
1 | PE/Cy7 anti-mouse CD3 | Biolegend | 100220 |
2 | APC anti-mouse CD4 | Biolegend | 100412 |
3 | Alexa Fluor® 700 anti-mouse CD25 | Biolegend | 102024 |
4 | PerCP/Cyanine5.5 anti-mouse CD8a | Biolegend | 100733 |
5 | Pacific Blueâ„¢ anti-mouse/human CD44 | Biolegend | 103020 |
6 | Brilliant Violet 605â„¢ anti-mouse CD45 | Biolegend | 103139 |
7 | PE/Dazzleâ„¢ 594 anti-human/mouse Granzyme B Recombinant | Biolegend | 372207 |
8 | Alexa Fluor® 488 anti-mouse FOXP3 | Biolegend | 126405 |
9 | PE anti-mouse H-2Dk | Biolegend | 110307 |
10 | Brilliant Violet 421â„¢ anti-mouse F4/80 | Biolegend | 123131 |
11 | PE/Dazzleâ„¢ 594 anti-mouse I-A/I-E | Biolegend | 107647 |
12 | FITC anti-mouse CD86 | Biolegend | 105005 |
13 | PE/Cy7 anti-mouse/human CD11b | Biolegend | 101215 |
14 | APC anti-mouse CD11c | Biolegend | 117309 |
15 | PerCP/Cyanine5.5 anti-mouse CD49b (pan-NK cells) | Biolegend | 108915 |
16 | Alexa Fluor® 700 anti-mouse Ly-6G/Ly-6C (Gr-1) | Biolegend | 108421 |
17 | PE/Cy7 Rat IgG2b, κ Isotype Ctrl | Biolegend | 400617 |
18 | APC Rat IgG2b, κ Isotype Ctrl | Biolegend | 400611 |
19 | PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl | Biolegend | 400531 |
20 | Pacific Blue™ Rat IgG2b, κ Isotype Ctrl | Biolegend | 400627 |
21 | Brilliant Violet 605™ Rat IgG2b, κ Isotype Ctrl | Biolegend | 400657 |
22 | Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl | Biolegend | 400625 |
23 | PE Mouse IgG2a, κ Isotype Ctrl | Biolegend | 400211 |
24 | Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl | Biolegend | 400549 |
25 | PE/Dazzle™ 594 Rat IgG2b, κ Isotype Ctrl | Biolegend | 400659 |
26 | FITC Rat IgG2a, κ Isotype Ctrl | Biolegend | 400505 |
27 | Alexa Fluor® 700 Rat IgG2b, κ Isotype Ctrl | Biolegend | 400628 |
28 | TruStain fcXâ„¢ (anti-mouse CD16/32) | Biolegend | 101320 |
29 | True-Nuclearâ„¢ Transcription Factor Buffer Set | Biolegend | 424401 |
1 | Penicillin–Streptomycin-Glutamine (100X) | Gibco | 10378016 |
2 | FBS | GIBCO | 10099–141 |
5 | DMEM | GIBCO | 10566–016 |
6 | Opti-MEM | Gibco | 31985070 |
7 | Trypsin–EDTA (0.25%), phenol red | Gibco | 25200072 |
8 | InVivoMab anti-mouse CD4 | BioXcell | BE0003-1-5MG |
9 | InVivoMab anti-mouse CD8 | BioXcell | BE0004-1-5MG |
10 | 100um cell strainer | Falcon | 352360 |
11 | 70um cell strainer | Falcon | 352350 |
12 | Percoll | GE Health | 17–0891-02 |
13 | DPBS | Hyclone | SH30028.02 |
14 | TLRL-1826 ODN | Invivogen | tlrl-1826–1 |
15 | Dimethyl sulfoxide (DMSO) | Sigma-Aldrich | D2650 |
16 | Collagenase A | Roche | 10103586001 |
17 | HBSS | Servicebio | G4204-500 |
18 | 10XPBS | Servicebio | G4207-500 |
19 | mouse IFN-gamma elispot plus(plus) HRP | MabTech | 3321-4HST-2 |
20 | Bovine Serum Albumin | SIGMA | V900933-100G |
21 | mouse IFN-gamma elispot plus(plus) HRP | MabTech | 3321-4HST-2 |
22 | CD45 MicroBeads, mouse | Miltenyi Biotec | 130–052-301 |
23 | Mouse IFN-gamma ELISpot PLUS (HRP), strips | MabTech | 3321-4HST-10 |
24 | EmbryoMaxâ„¢ Nucleosides (100X) | merck | ES-008 |