Fig. 3From: Assembling Au8 clusters on surfaces of bifunctional nanoimmunomodulators for synergistically enhanced low dose radiotherapy of metastatic tumora Immunofluorescence checking was shown and verified the specific localization of HMGB1 before and after irradiation. b Relationship between radiotherapy dose and ATP concentration released by tumor cells. c Representative images of the colony formation assay of 4T1 cells in R837/BMS@Au8 NP and R837/BMS@Au8 NPs + RT groups. d Numbers of clonogenity in the two groups. Data are presented as mean ± SD. Statistical significance was determined using the two tailed Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001 in comparison with control or G1 group, n = 3Back to article page