Fig. 2

Evaluation of the potentiating effect of AAV-14D5-dimHLE vector on the activity of P2X7 following i.m. administration. AAV-14D5-dimHLE vector was injected i.m. at a dose of 10¹¹ vg/mouse. Blood cells were collected 21 days later and the functional activity of P2X7 on the surface of T cell subsets was evaluated after incubation with 30 µM or 150 µM ATP. Flow cytometry profiles of CD62L expression (A) and percentages (B) of CD4⁺CD27⁺CD62L^high cells in the gated CD8⁺, CD4⁺CD25⁻, or CD4⁺CD25⁺ (Tregs) subsets are shown. One representative experiment out of at least two is shown with n = 3 mice per experiment