Fig. 3

Evaluation of the blocking effect of AAV-13A7-Fc vector on the activity of P2X7 following i.m. administration of different doses of AAVnano vector. AAV-13A7-Fc vector was injected i.m. at the indicated AAVnano dose and blood cells were collected 15 and 21 days later to evaluate the functional activity of P2X7 on the surface of CD4⁺ T cells upon incubation with 150 µM ATP for 15 min. A Representative flow cytometry panels illustrating the ATP-induced P2X7-dependant CD62L shedding within gated CD4⁺ T cells, 15 days post-AAV injection (numbers correspond to the percentages of CD4⁺ CD62L^high cells). The percentages obtained in the absence of ATP is 86% (not shown). B Percentages of CD4⁺ CD62L^high cells in each experimental condition, from blood cells collected 15 or 21 days post-AAV injection, after treatment ex vivo with 150 µM ATP for 15 min. Results represent mean values ± SEM, with n = 5 mice per group. *p < 0.05, ***p < 0.001 two-way ANOVA