Fig. 8

Chemokine and proinflammatory cytokine production was reduced in mice receiving AAV-13A7-Fc. Mice (n = 10) receiving AAVnano coding for 13A7-Fc or 14D5-dimHLE at a dose of 10¹¹ vg/mouse, were given, three weeks later, 2.5% DSS in drinking water for five days. Colons were collected on day 13 for chemokine and cytokine assessment. A mRNA expression of multiple chemokines (CXCL9, CXC10 and CCL2) and cytokines (IFNγ, IL-1β, IL-6, IL-17, IL-18 and IL-22) in the colons of mice injected with AAV coding for 13A7-Fc or 14D5-dimHLE and exposed to DSS (day 13 from the beginning of DSS treatment). B IL-1 β and IL-18 levels in the supernatants of colon explants after 16 h of culture. Results represent the combined data of two different experiments normalized to the DSS-treated group expression values. Statistical analysis was performed by one-way ANOVA with *p < 0.05, **p < 0.01 and ***p < 0.001